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|Title:||Astragaloside IV and cycloastragenol production in in vitro produced Astragalus trojanus Stev.|
Akgün, Ismail Hakki
|Keywords:||Astragalus trojanus;Astragaloside IV;Cycloastragenol;Multiple shoot proliferation;Rooting|
|Abstract:||In the present paper, an efficient method for clonal propagation of an endemic plant, Astragalus trojanus Stev., was described. Effect of sucrose and 6-benzyladenine (BA) concentrations on shoot proliferation and rooting was studied. Woody plant medium (WPM) supplemented with two different concentrations of BA (S1 medium with 0.5 mg/L BA & S2 medium with 1 mg/L BA) was used, where S2 medium resulted in higher rates in both shoot elongation and multiple shoot proliferation of clonally propagated A. trojanus shoot cultures. Of 27 clones, 3 were selected for further studies based on the highest regeneration capacity (Clones 1, 12 & 15). A total of 340 shoots were obtained from Clone 1 in S1 medium. In this medium, the rooting ratio of shoots for Clone 15 was 11.67%. The highest shoot elongation (1.99 cm), multiple shoot proliferation ratio (4.35) and multiple shoot proliferation percentage (91.67%) were obtained from Clone 1 in S2 medium. Two media, WPM medium supplemented with 15 g/L (K1 medium) and 30 g/L (K2 medium) sucrose were used for rooting. The root formation of Clone 1 was 26.67% in K2 medium. The highest astragaloside IV (0.5486 µg/mg) and cycloastragenol (0.1263 µg/mg) contents were detected in roots grown in K1 medium.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.14(4) [October 2015]|
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