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|Title:||Functional gene based multiplex PCR to detect bacteria implicated in biotransformation of nitrogen, sulfur and methane in aquatic environment|
|Authors:||Krishnani, Kishore Kumar|
|Keywords:||Biotransformation;Coastal aquaculture;Functional genes;Metagenomics;Multiplex PCR|
|Abstract:||Nitrifying, denitrifying, sulfur oxidizing and methane oxidizing bacteria have significant potential for bioremediation, which would require methods for monitoring the presence of these organisms in aquatic and related environmental samples. This study provides a functional gene multiplex PCR (M-PCR) for detection of microorganisms capable of biotransformation of nitrogenous and sulfurous metabolites, such as, ammonia, nitrite, nitrate and sulfide, and potent greenhouse gases, such as, nitric oxide, nitrous oxide and methane. Oligonucleotide primers have been developed based on the sequences obtained through the creation of metagenomic clone libraries and available sequence data. Clones obtained were used as positive templates. M-PCR methods are accurate and sensitive in detection of these organisms in a variety of environmental and bacterial samples collected from aquatic environments. This has an application in more accurate understanding of aquatic metabolites in coastal aquaculture and related environment.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.14(4) [October 2015]|
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