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Title: In silico EST mining of Fabaceae species
Authors: Bhati, Jyotika
Chandrasekaran, H
Chand, Suresh
Keywords: ESTs;Fabaceae;Functional annotation;GC content;SSR
Issue Date: Oct-2015
Publisher: NISCAIR-CSIR, India
Abstract: The Fabaceae family, one of the most extensively studied plant lectin families, has received increasing attention for the remarkable property of fixing nitrogen from the atmosphere. Legumes are one of a few plant families with extensive genomic data available in multiple species. The unprecedented complexity and impending completeness of these data create opportunities for new approaches to discovery. In the present study, 24,749 ESTs of 5 Fabaceae species were clustered into unigenes contigs and compared with Medicago truncatula gene indices. Almost all the unigenes of Fabaceae species showed high similarity with Medicago genes, except those of Lens culinaris where only 95% of unigenes were found similar. A total of 315 SSRs were identified in the unigenes. Functional annotation of unigenes showed that the majority of the genes are present in metabolism and energy functional classes. Given the progress in the development of genomics in the model legume M. truncatula, there is a major scientific opportunity to translate the genomic advances from this species to other less studied legumes. This would be helpful for genomics as well as evolutional studies, and DNA markers developed can be used for mapping, tagging and cloning of important trait specific genes in Fabaceae.
Page(s): 461-468
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(4) [October 2015]

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