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Title: Genetic uniformity analysis of cryopreseved in vitro plantlets of Kaempferia galanga L.—An endangered medicinal species in Tropical Asia
Authors: Preetha, T S
Kumar, A S Hemantha
Padmesh, P
Krishnan, P N
Keywords: Cryopreservation;Kaempferia galanga;RAPD;Shoot tip;Somatic embryo
Issue Date: Jul-2015
Publisher: NISCAIR-CSIR, India
Abstract: While using the protocols for the long-term conservation, assessment of true-to-type regenerants from cryopreserved materials is important. In the present study, no significant variation in banding pattern was noticed in the RAPD profile of cryopreserved and control samples of shoot tip-derived Kaempferia galanga plants. But in the case of somatic embryo-derived samples, some variation was observed in the banding pattern (16.2% polymorphism), though the plants were phenotypically similar. Recovery of cryopreserved somatic embryos in K. galanga was preceded through a callus phase, from which secondary embryos were induced later. The RAPD profile of somatic embryo-derived samples revealed that variation was due to the occurrence of callus phase. As minor genetic variations arising in in vitro cultures without marked phenotypic changes are considered to be beneficial for diversity conservation and sustainable utilization, cryopreserved somatic embryos of K. galanga would serve as an alternative for generating and maintaining genetic diversity of this medicinal wealth.
Page(s): 425-428
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(3) [July 2015]

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