Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/33414
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dc.contributor.authorGauthami, S-
dc.contributor.authorMeena, Keerti-
dc.contributor.authorKumar, Deepak-
dc.contributor.authorUma, Madala-
dc.contributor.authorReddy, Y Vishnuvardhan-
dc.contributor.authorRao, Pavuluri Panduranga-
dc.contributor.authorHegde, Nagendra R-
dc.date.accessioned2015-11-23T10:31:28Z-
dc.date.available2015-11-23T10:31:28Z-
dc.date.issued2015-07-
dc.identifier.issn0975-0967 (Online); 0972-5849 (Print)-
dc.identifier.urihttp://hdl.handle.net/123456789/33414-
dc.description376-381en_US
dc.description.abstractA method to prepare a concentrated monophasic phenol guanidinium isothiocyanate-based reagent for extraction of RNA from liquid samples is described. The reagent was comparable to a commercial reagent for extraction of genetic material of several RNA viruses as assessed by limit of detection of RNA by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The study showed that the concentrated monophasic reagent could be used in place of a commercial reagent.en_US
dc.language.isoen_USen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBT Vol.14(3) [July 2015]en_US
dc.subjectAcid phenolen_US
dc.subjectRNA isolationen_US
dc.subjectRT-PCRen_US
dc.subjectVirus R NAen_US
dc.titleMPGIT: A concentrated monophasic phenol-guanidinium isothiocyanate reagent for isolation of viral RNAen_US
dc.typeArticleen_US
Appears in Collections:IJBT Vol.14(3) [July 2015]

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