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Title: MPGIT: A concentrated monophasic phenol-guanidinium isothiocyanate reagent for isolation of viral RNA
Authors: Gauthami, S
Meena, Keerti
Kumar, Deepak
Uma, Madala
Reddy, Y Vishnuvardhan
Rao, Pavuluri Panduranga
Hegde, Nagendra R
Keywords: Acid phenol;RNA isolation;RT-PCR;Virus R NA
Issue Date: Jul-2015
Publisher: NISCAIR-CSIR, India
Abstract: A method to prepare a concentrated monophasic phenol guanidinium isothiocyanate-based reagent for extraction of RNA from liquid samples is described. The reagent was comparable to a commercial reagent for extraction of genetic material of several RNA viruses as assessed by limit of detection of RNA by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The study showed that the concentrated monophasic reagent could be used in place of a commercial reagent.
Page(s): 376-381
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(3) [July 2015]

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