Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/3304
Title: Distribution and biodegradation of polycyclic aromatic hydrocarbons in contaminated sites of Hisar (India)
Authors: Bishnoi, Kiran
Sain, Umashanker
Kumar, Rajender
Singh, Rajesh
Bishnoi, Narsi R
Keywords: Biodegradation
Phenanthrene
Polycyclic aromatic hydrocarbons
Pseudomonas paucimobilis
Pseudomonas putida
Pyrene
Issue Date: Mar-2009
Publisher: CSIR
Abstract: Fifty-two soil samples were collected from various location of the Hisar city. These samples were analysed for six polycyclic aromatic hydrocarbons (naphthalene, acenaphthene, phenanthrene, anthracene, fluoranthene, and pyrene). Total mean concentration of six PAHs varied from 51.79 to 148.82 mg kg⁻¹ dry weight of the soil, PAH concentration was higher in soil samples from local auto market while lower concentration was recorded in agricultural soil. Effect of pH (5.0 to 9.0), temperature (20º to 40ºC), and concentration of PAHs (5 to 20 mg kg⁻¹) on biodegradation were optimized. Biodegradation of phenanthrene (3-ring) and pyrene (4-ring) was evaluated using two acclimatized microbial strains Pseudomonas putida and Pseudomonas paucimobilis. Biodegradation was maximum in sterilized artificial spiked soil with phenanthrene (5 mg kg⁻¹) and pyrene (5 mg kg⁻¹) at pH 7.0 and at 30ºC (optimized conditions) than the native unsterilized contaminated soil (without optimized conditions) in 42 days of incubation period with Pseudomonas putida and Pseudomonas paucimobilis. Phenanthrene was completely disappeared after 28 days with P. putida and after 35 days with P. paucimobilis. Whereas, pyrene was disappeared up to 97.40 % with P. putida and 95.5% with P. paucimobilis after 42 days incubation period at optimum conditions. Under unoptimized conditions, disappearance of phenanthrene was 65.89% with P. putida and 57.81% with P. paucimobilis after 42 days, whereas the % disappearance of pyrene was 59.80% with P. putida and 52.07% with P. paucimobilis.
Description: 210-217
URI: http://hdl.handle.net/123456789/3304
ISSN: 0019-5189
Appears in Collections:IJEB Vol.47(03) [March 2009]

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