Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/33018
Title: Evaluation of food-drug interaction potential of Aegle marmelos (L.) Corrêa through metabolism mediated cytochrome P450 inhibition assay
Authors: Bahadur, Shiv
Mukherjee, Kakali
Kar, Amit
Ahmmed, SK Milan
Mukherjee, Pulok K
Al-Dhabi, Naif Abdullah
Duraipandiyan, V
Keywords: Cytochrome P450;RP-HPLC;Imperatorin;Aegle marmelos (L.) Corrêa
Issue Date: Oct-2015
Publisher: NISCAIR-CSIR, India
IPC Code: Int. Cl.8: A61K 36/00, A61K, A47G 19/26, A47J 39/02, C12N 9/00, C12N 11/00
Abstract: In the present study, possible cytochrome enzyme inhibition potential of Aegle marmelos (L.) Corrêa fruit and its bioactive compound was evaluated. Interaction potential of Aegle marmelos extract was evaluated through CYP450-carbonmonoxide complex (CYP450-CO) assay. Additionally, influence on individual major human cytochrome P450 drug metabolizing enzymes such as CYP3A4, CYP2D6, CYP2C9 and CYP1A2 were studied through fluorescence screening assay. Bioactive compound was quantified through RP-HPLC, in order to standardize the sample material. Aegle marmelos extract showed higher IC50 value than the respective standard inhibitors and lower than the bioactive compound imperatorin. Aegle marmelos extract showed significantly lesser (P<0.001, P<0.01) interaction potential than the standard inhibitors. The research findings indicated that selected sample is unlikely to cause clinically relevant drug interactions involving inhibition of major CYP isozymes.
Page(s): 643-649
URI: http://hdl.handle.net/123456789/33018
ISSN: 0975-1068 (Online); 0972-5938 (Print)
Appears in Collections:IJTK Vol.14(4) [October 2015]

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