NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.47 [2009] >
IJEB Vol.47(03) [March 2009] >


Title: In vitro growth and shoot multiplication in Terminalia bellerica Roxb. under controlled carbon dioxide environment
Authors: Suthar, R K
Rathore, P
Purohit, S D
Keywords: Carbon dioxide enrichment
Medicinal plant
Photoautotrophic multiplication
Issue Date: Mar-2009
Publisher: CSIR
Abstract: The shoot cultures of Terminalia bellerica Roxb. were grown on Murashige and Skoog’s medium containing 1.5 mg l ⁻¹ BAP (6- benzyl aminopurine), and supplemented with or without sucrose (3 %). A range of CO₂ concentrations (0.0, 0.6, 10, 40 g⁻³) was provided in small acrylic chambers by using different concentrations and combinations of NaHCO₃ (sodium bicarbonate), Na₂CO₃ (sodium carbonate), KHCO₃ (potassium bicarbonate) and K₂CO₃ (potassium carbonate). To obtain a CO₂-free environment, a saturated solution of 10 % of KOH (potassium hydroxide) was kept in the chamber. Complete absence of carbon source caused death of shoots within 20 days. Under controlled and enriched CO2, the shoots grew fully photoautotropically on sucrose-free medium. The growth of cultures was better with carbon dioxide (40 g⁻³) than sucrose (3%) in the medium. Maximum number of shoots, number of leaves per cluster, fresh and dry weight and chlorophyll contents were recorded when both sucrose and CO₂ (40 g⁻³) were provided to the culture.
Page(s): 204-209
ISSN: 0019-5189
Source:IJEB Vol.47(03) [March 2009]

Files in This Item:

File Description SizeFormat
IJEB 47(3) 204-209.pdf414 kBAdobe PDFView/Open
 Current Page Visits: 649 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 602448 since 06-Feb-2009  Last updated on 21-Oct-2014Webmaster: nopr@niscair.res.in