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IJEB Vol.47(03) [March 2009] >


Title: Role of PGRs and inhibitors in induction and control of somatic embryogenesis in Themeda quadrivalvis
Authors: Habibi, N
Suthar, R K
Purohit, S D
Keywords: Plant growth regulators
Plant inhibitors
Somatic embryogenesis
Themeda quadrivalvis
Issue Date: Mar-2009
Publisher: CSIR
Abstract: Somatic embryogenesis could be achieved in Themeda quadrivalvis (Linn.) O. Ktze -fodder grass species on MS medium supplemented with 2,4-D. Incorporation of putrescine in the medium stimulated embryogenesis, however its lower concentration stimulated production of non-regenerative callus. Other polyamines such as spermine and spermidine could not evoke similar response. Ascorbic acid used as antioxidant could not prevent browning in embryogenic cultures, however it stimulated embryogenesis. Inhibition of auxin polar transport by use of TIBA and HFCA reduced the embryogenic response significantly and produced distorted or abnormal embryos. Antiethylene substances such as AgNO₃ and CoCl₂ added in the medium adversely affected the process of embryogenesis and counteracting the stimulatory role of ethylene.
Page(s): 198-203
ISSN: 0019-5189
Source:IJEB Vol.47(03) [March 2009]

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