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Title: Isolation of stress responsive Psb A gene from rice (Oryza sativa L.) using differential display
Authors: Tyagi, Aruna
Chandra, Arti
Keywords: Gene;Drought stress;Rice;Oryza sativa L.
Issue Date: Aug-2006
Publisher: CSIR
Abstract: Differential display (DD) experiments were performed on drought-tolerant rice (Oryza sativa L.) genotype N22 to identify both upregulated and downregulated partial cDNAs with respect to moisture stress. DNA polymorphism was detected between drought-stressed and control leaf tissues on the DD gels. A partial cDNA showing differential expression, with respect to moisture stress was isolated from the gel. Northern blotting analysis was performed using this cDNA as a probe and it was observed that mRNA corresponding to this transcript was accumulated to high level in rice leaves under water deficit stress. At the DNA sequence level, the partial cDNA showed homology with psb A gene encoding for D1 protein.
Page(s): 244-246
ISSN: 0301-1208
Appears in Collections:IJBB Vol.43(4) [August 2006]

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