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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.43 [2006] >
IJBB Vol.43(4) [August 2006] >
| Title: | Isolation of stress responsive Psb A gene from rice (Oryza sativa L.) using differential display |
| Authors: | Tyagi, Aruna
Chandra, Arti |
| Keywords: | Gene
Drought stress
Rice
Oryza sativa L. |
| Issue Date: | Aug-2006 |
| Publisher: | CSIR |
| Abstract: | Differential display (DD) experiments were performed on drought-tolerant rice (Oryza sativa L.) genotype N22 to identify both upregulated and downregulated partial cDNAs with respect to moisture stress. DNA polymorphism was detected between drought-stressed and control leaf tissues on the DD gels. A partial cDNA showing differential expression, with respect to moisture stress was isolated from the gel. Northern blotting analysis was performed using this cDNA as a probe and it was observed that mRNA corresponding to this transcript was accumulated to high level in rice leaves under water deficit stress. At the DNA sequence level, the partial cDNA showed homology with psb A gene encoding for D1 protein. |
| Page(s): | 244-246 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.43(4) [August 2006]
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