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http://nopr.niscair.res.in/handle/123456789/3283
Title: | Purification of a peroxidase from Solanum melongena fruit juice |
Authors: | Vernwal, S K Yadav, R S S Yadav, K D S |
Keywords: | Peroxidase;Solanum melongena;Fruit |
Issue Date: | Aug-2006 |
Publisher: | CSIR |
Abstract: | Solanum melongena fruit juice contains peroxidase activity of the order of 0×125 IU/mL. A method for the 11-fold purification of the enzyme was developed. The Km values of the peroxidase for the substrates guaiacol and hydrogen peroxide were 6×5 mM and 0×33 mM, respectively. The pH and temperature optima were 5×5 and 84°C, respectively using guaiacol as the substrate. Sodium azide and phenyl hydrazine inhibited the enzyme competitively. |
Page(s): | 239-243 |
URI: | http://hdl.handle.net/123456789/3283 |
ISSN: | 0301-1208 |
Appears in Collections: | IJBB Vol.43(4) [August 2006] |
Files in This Item:
File | Description | Size | Format | |
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IJBB 43(4) 239-243.pdf | 308.1 kB | Adobe PDF | View/Open |
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