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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.43 [2006] >
IJBB Vol.43(4) [August 2006] >
| Title: | Purification of a peroxidase from Solanum melongena fruit juice |
| Authors: | Vernwal, S K
Yadav, R S S
Yadav, K D S |
| Keywords: | Peroxidase
Solanum melongena
Fruit |
| Issue Date: | Aug-2006 |
| Publisher: | CSIR |
| Abstract: | Solanum melongena fruit juice contains peroxidase activity of the order of 0×125 IU/mL. A method for the 11-fold purification of the enzyme was developed. The Km values of the peroxidase for the substrates guaiacol and hydrogen peroxide were 6×5 mM and 0×33 mM, respectively. The pH and temperature optima were 5×5 and 84°C, respectively using guaiacol as the substrate. Sodium azide and phenyl hydrazine inhibited the enzyme competitively. |
| Page(s): | 239-243 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.43(4) [August 2006]
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