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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.43 [2006] >
IJBB Vol.43(3) [June 2006] >
| Title: | Regulation of MAL1⁺ gene expression encoding maltase in Schizosaccharomyces pombe by added inositol |
| Authors: | Yao, Shumin
Chi, Zhenming
He, Susu |
| Keywords: | Schizosaccharomyces pombe
Phosphatidylinositol
Derepression
MAL1⁺ gene encoding maltase
Inositol |
| Issue Date: | Jun-2006 |
| Publisher: | CSIR |
| Abstract: | In this study, the effects of inositol addition on maltase activity and expression of MAL1⁺ gene encoding maltase in Schizosaccharomyces pombe were investigated. The maximum specific maltase activity was observed, when the concentration of inositol reached 6.0 μg/ml in the synthetic medium containing 2.0% glucose. At 1.0 μg/ml inositol concentration, the maltase activity continuously decreased, as initial glucose concentration was higher than 0.1%. mRNA encoding maltase and phosphatidylinositol (PI) content were higher in the cells grown in the synthetic medium with 6.0 μg/ml of inositol and 2.0% glucose than those with 1.0 μg/ml of inositol. These results demonstrated that higher inositol concentration in the synthetic medium could derepress MAL1⁺ gene expression in S. pombe and PI might be involved in derepression of MAL1⁺ gene expression in S. pombe probably by PI-type signalling pathway. |
| Page(s): | 143-147 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.43(3) [June 2006]
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