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|Title:||Regulation of MAL1⁺ gene expression encoding maltase in Schizosaccharomyces pombe by added inositol|
|Keywords:||Schizosaccharomyces pombe;Phosphatidylinositol;Derepression;MAL1⁺ gene encoding maltase;Inositol|
|Abstract:||In this study, the effects of inositol addition on maltase activity and expression of MAL1⁺ gene encoding maltase in Schizosaccharomyces pombe were investigated. The maximum specific maltase activity was observed, when the concentration of inositol reached 6.0 μg/ml in the synthetic medium containing 2.0% glucose. At 1.0 μg/ml inositol concentration, the maltase activity continuously decreased, as initial glucose concentration was higher than 0.1%. mRNA encoding maltase and phosphatidylinositol (PI) content were higher in the cells grown in the synthetic medium with 6.0 μg/ml of inositol and 2.0% glucose than those with 1.0 μg/ml of inositol. These results demonstrated that higher inositol concentration in the synthetic medium could derepress MAL1⁺ gene expression in S. pombe and PI might be involved in derepression of MAL1⁺ gene expression in S. pombe probably by PI-type signalling pathway.|
|Appears in Collections:||IJBB Vol.43(3) [June 2006]|
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