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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.43 [2006] >
IJBB Vol.43(2) [April 2006] >
| Title: | Effect of endosulfan on growth, ⍺ amylase activity and plasmids amplification in Bacillus subtilis |
| Authors: | Tolan, Veysel
Ensari, Yavuz |
| Keywords: | Endosulfan
Bacillus subtilis
α amylase
Plasmid |
| Issue Date: | Apr-2006 |
| Publisher: | CSIR |
| Abstract: | Endosulfan, a chlorinated hydrocarbon insecticide of cyclodiene subgroup acts as a contact poison in a wide variety of organisms. In the present study, the effect of endosulfan on the growth, α amylase activity and plasmid amplification was investigated in Bacillus subtilis system. The bacteria were grown in medium, incubated with different concentrations (32, 48, 64 and 80 μg/mL) of endosulfan. The bacterial growth was gradually seen after 1st day at up to 48 μg/L endosulfan. The 48 μg/L endosulfan inhibited approximately 50% of the bacterial growth. No growth was observed at and after 64 μg/L endosulfan, for all days (1-5). Also, no ⍺ amylase activity was found in the supernatant of the culture medium containing 64 and 80 μg/L endosulfan, whereas slight activity was observed with 32 and 48 μg/L endosulfan concentration. The amount of plasmid increased up to 50% in the presence of 32 μg/L endosulfan. Endosulfan had no effect on the ⍺ amylase activity in vitro. |
| Page(s): | 123-126 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.43(2) [April 2006]
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