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IJBB Vol.43(2) [April 2006] >


Title: Effect of endosulfan on growth, ⍺ amylase activity and plasmids amplification in Bacillus subtilis
Authors: Tolan, Veysel
Ensari, Yavuz
Keywords: Endosulfan
Bacillus subtilis
α amylase
Plasmid
Issue Date: Apr-2006
Publisher: CSIR
Abstract: Endosulfan, a chlorinated hydrocarbon insecticide of cyclodiene subgroup acts as a contact poison in a wide variety of organisms. In the present study, the effect of endosulfan on the growth, α amylase activity and plasmid amplification was investigated in Bacillus subtilis system. The bacteria were grown in medium, incubated with different concentrations (32, 48, 64 and 80 μg/mL) of endosulfan. The bacterial growth was gradually seen after 1st day at up to 48 μg/L endosulfan. The 48 μg/L endosulfan inhibited approximately 50% of the bacterial growth. No growth was observed at and after 64 μg/L endosulfan, for all days (1-5). Also, no ⍺ amylase activity was found in the supernatant of the culture medium containing 64 and 80 μg/L endosulfan, whereas slight activity was observed with 32 and 48 μg/L endosulfan concentration. The amount of plasmid increased up to 50% in the presence of 32 μg/L endosulfan. Endosulfan had no effect on the ⍺ amylase activity in vitro.
Page(s): 123-126
ISSN: 0301-1208
Source:IJBB Vol.43(2) [April 2006]

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