NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.43 [2006] >
IJBB Vol.43(2) [April 2006] >


Title: Ficus cunia agglutinin for recognition of bacteria
Authors: Adhya, M
Singha, B
Chatterjee, B P
Keywords: Ficus cunia
Agglutinin
Bacteria
ELISA
Issue Date: Feb-2006
Publisher: CSIR
Abstract: Interaction of bacteria with lectin using anti-lectin antibody by ELISA is an established method. In the present study, we have devised a simple ELISA using a biotinylated lectin and antibiotin-HRP. Ficus cunia agglutinin (FCA), which has shown the specificity towards / anomers of GlcNAc and other –NAc containing sugars like LacNAc and GlcNAc β(1-4/6)GlcNAc, was used as a model lectin for the study of interaction with immobilized microorganisms on ELISA plate. The bacterial cells of E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis and Staphylococcus aureus showed binding with FCA and the degree of binding was dependent on the bacterial surface antigen. This method is considered a simple technique to study the lectin-bacteria interaction.
Page(s): 94-97
ISSN: 0301-1208
Source:IJBB Vol.43(2) [April 2006]

Files in This Item:

File Description SizeFormat
IJBB 43(2) 94-97.pdf79.01 kBAdobe PDFView/Open
 Current Page Visits: 623 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 624636 since 06-Feb-2009  Last updated on 13-Nov-2014Webmaster: nopr@niscair.res.in