NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.43 [2006] >
IJBB Vol.43(1) [February 2006] >

Title: Kinetics and mechanism of reduction of ferricytochrome c by glutathione and L-cysteine: A comparative study
Authors: Subudhi, U
Chainy, G B N
Mohanty, P
Keywords: Ferricytochrome c
Issue Date: Feb-2006
Publisher: CSIR
IPC CodeC07K14/80
Abstract: The kinetics and mechanism of the reduction of ferricytochrome c [Cyt c(III)] by substrates namely glutathione (GSH) and L-cysteine (L-cys) have been investigated spectrophotometrically employing [substrate]T >> [Cyt c(III)]T. The reaction exhibits first order dependence in [substrate]T and [Cyt c(III)]T. The pseudo-first order rate constant increases with an increase in pH, indicating that the conjugate base form of the HCyt c(III) is a better oxidant than the parent HCyt c(III). The electron transfer rate constants between the oxidants and GSH for both the k₁ and k₂ paths are found to be greater than that with L-cysteine. Hence, GSH is a better reductant of Cyt c(III) as compared to L-cysteine. A suitable mechanism has been proposed on the basis of experimental findings. The deprotonation constant for HCyt c(III) and the second order rate constants of k₁ and k₂ paths for the present reaction at 25ºC have been determined.
Page(s): 37-40
ISSN: 0301-1208
Source:IJBB Vol.43(1) [February 2006]

Files in This Item:

File Description SizeFormat
IJBB 43(1) 37-40.pdf80.27 kBAdobe PDFView/Open
 Current Page Visits: 83 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 171234 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: