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Title: Antioxidant potential of C-phycocyanin isolated from cyanobacterial species Lyngbya, Phormidium and Spirulina spp.
Authors: Patel, Anamika
Mishra, Sandhya
Ghosh, P K
Keywords: C-Phycocyanin
Hydroxyl and peroxyl radical scavenger
Electron spin resonance
Issue Date: Feb-2006
Publisher: CSIR
IPC CodeA61K35/74
Abstract: The antioxidant activity of C-Phycocyanin (C-PC) isolated from three cyanobacterial species Lyngbya (marine), Phormidium (marine) and Spirulina (fresh water) was studied in vitro. The results demonstrate that C-PCs from Lyngbya, Phormidium and Spirulina spp. are able to scavenge peroxyl radicals (determined by crocin bleaching assay) with relative rate constant ratio of 3.13, 1.89 and 1.8, respectively. C-PCs also scavenge hydroxyl radicals (determined by deoxyribose degradation assay) with second order rate constant values of 7.87  10¹⁰, 9.58  10¹⁰ and 6.42  10¹⁰, respectively. Interestingly, Lyngbya C-PC is found to be an effective inhibitor of peroxyl radicals (IC₅₀ 6.63 μM), as compared to Spirulina (IC₅₀ 12.15 μM) and Phormidium C-PC (IC₅₀ 12.74 μM) and is close to uric acid (IC₅₀ 2.15 μM). Further, the studies suggest that the covalently-linked tetrapyrrole chromophore phycocyanobilin is involved in the radical scavenging activity of C-PC. The electron spin resonance (ESR) spectra of C-PCs indicate the presence of free radical active sites, which may play an important role in its radical scavenging property. This is the first report on the ESR activity of native C-PCs without perturbations that can cause radical formation.
Page(s): 25-31
ISSN: 0301-1208
Source:IJBB Vol.43(1) [February 2006]

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