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dc.contributor.authorJames, Donald-
dc.contributor.authorTarafdar, Avijit-
dc.contributor.authorBiswas, Koushik-
dc.contributor.authorSathyavathi, Tara C-
dc.contributor.authorPadaria, Jasdeep Chatrath-
dc.contributor.authorKumar, P Ananda-
dc.identifier.issn0975-1009 (Online); 0019-5189 (Print)-
dc.description.abstractPearl millet (Pennisetum glaucum L. R. Br.) is an important cereal crop grown mainly in the arid and semi-arid regions of India known to possess the natural ability to withstand thermal stress. To elucidate the molecular basis of high temperature response in pearl millet, 12 days old seedlings of P. glaucum cv. 841A were subjected to heat stress at 46°C for different time durations (30 min, 2, 4, 8, 12 and 24 h) and a forward subtractive cDNA library was constructed from pooled RNA of heat stressed seedlings. A total of 331 high quality Expressed Sequence Tags (ESTs) were obtained from randomly selected 1050 clones. Sequences were assembled into 103 unique sequences consisting of 37 contigs and 66 singletons. Of these, 92 unique sequences were submitted to NCBI dbEST database. Gene Ontology through RGAP data base and BLASTx analysis revealed that about 18% of the ESTs showed homology to genes for “response to abiotic and biotic stimulus”. About 2% of the ESTs showed no homology with genes in dbEST, indicating the presence of uncharacterized candidate genes involved in heat stress response in P. glaucum. Differential expression of selected genes (hsp101 and CRT) from the SSH library were validated by qRT-PCR analysis. The ESTs thus generated are a rich source of heat stress responsive genes, which can be utilized in improving thermotolerance of other food crops. en_US
dc.publisherNISCAIR-CSIR, India-
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJEB Vol.53(08) [August 2015]en_US
dc.subjectExpressed sequence tagsen_US
dc.subjectHeat stressen_US
dc.subjectHeat shock proteinsen_US
dc.subjectqPCR validationen_US
dc.subjectSSH libraryen_US
dc.subjectStress responsive genesen_US
dc.subjectThermal stressen_US
dc.titleDevelopment and characterization of a high temperature stress responsive subtractive cDNA library in Pearl Millet Pennisetum glaucum (L.) R.Br.en_US
Appears in Collections:IJEB Vol.53(08) [August 2015]

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