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Title: Optimization of inoculum density in hairy root culture of Plumbago rosea L. for enhanced growth and plumbagin production towards scaling-up in bioreactor
Authors: Pillai, Dhanya B
Jose, Binoy
Satheeshkumar, K
Krishnan, P N
Keywords: Growth index;Inoculum density (ID);Plumbagin;Plumbago rosea L.
Issue Date: Apr-2015
Publisher: NISCAIR-CSIR, India
Abstract: The present study evaluated the influence of initial inoculum density (ID) on growth and plumbagin production in hairy root culture of Plumbago rosea L. in shake-flask as well as air-sparged bioreactor. IDs ranging from that of single root tip (0.08 g/L) to 10 g/L were analyzed in 50 mL MS basal media of 250 mL Erlenmeyer flasks. For scaling-up in bioreactor of 2 L working-capacity, 0.5, 1, 5, 10 and 15 g/L IDs were analysed. Optimal ID was found to be 1 g/L in shake-flask culture, producing a growth index of 36.15 on 30th d with plumbagin content of 1.325 g in 100 g DW of roots (1.325%). In the bioreactor, biomass increase was maximum for 5 g/L with a growth index of 11.428 and plumbagin content of 1.425%. Here we report the maximum plumbagin concentration (1.325%) in P. rosea hairy root culture, enhanced from 0.99% innate plumbagin content of the mother culture through sole optimization of ID. Further enhancement to 1.425% was attained by scaling-up in the bioreactor. The plumbagin concentration obtained in bioreactor culture of P. rosea hairy roots (1.425%) is also the highest reported so far in scaling-up. Thus results indicate that ID is a critical parameter influencing enhanced production of root biomass and plumbagin in hairy root cultures of P. rosea.
Page(s): 264-269
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(2) [April 2015]

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