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Title: Relative expression profile of lingual antimicrobial peptide (LAMP) mRNA by different tissues of Bubalus bubalis
Authors: Kalita, Dhruba Jyoti
Keywords: Cycle threshhold;Lingual antimicrobial peptide (LAMP);Relative expression;mRNA
Issue Date: Apr-2015
Publisher: NISCAIR-CSIR, India
Abstract: Lingual antimicrobial peptide (LAP) is a member of the β-defensin family and predominantly expressed by the tongue epithelium. Present study was conducted to investigate the relative expression of LAP mRNA by different tissues of Bubalus bubalis. Different tissues, viz., tongue, testis, uterus, liver and myeloids from bone were collected from freshly slaughtered healthy animal. Total RNA was extracted and cDNA was synthesized. Relative expression of LAP mRNA by different tissues was studied using real-time PCR with reference to buffalo β-actin gene. Cycle threshold (Ct) values were determined and ΔCt value for each tissue was estimated. The tongue epithelia showed the highest level of LAP gene expression (ΔCt -11.46), followed by uterus (ΔCt -11.27), testis (ΔCt -8.16), liver (ΔCt -4.89) and myeloid tissues (ΔCt 0.10). From the present study it can be concluded that B. bubalis LAP is constitutively expressed by different tissues.
Page(s): 256-259
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(2) [April 2015]

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