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Title: Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system
Authors: Kaushal, Manoj
Kaushal, Rajesh
Keywords: Acetylene reduction assay (ARA);Bacillus spp.;Biocontrol;Cauliflower;INM;PGPR;16S rRNA
Issue Date: Apr-2015
Publisher: NISCAIR-CSIR, India
Abstract: Isolates from rhizosphere were screened and characterized for their plant growth promotion and biocontrol properties as per standard methods. Five isolates (MK2, MK4, MK5, MK7 & MK9) showed the maximum plant growth promoting attributes like P-solubilization, N-fixation etc. Acetylene reduction assay (ARA) method was used to study N2 fixing ability. The bacterial isolate MK5 fixed the maximum nitrogen, i.e., 437.26 ηmole C2H4 h-1 mg-1 protein (109 mg of N2 fixed/ha/d). The isolates MK5 and MK7 showed the maximum homology with Bacillus pumilus and B. subtilis, respectively by 16S rRNA analysis. The bacterial isolate MK5 in conjunction with 75% level of recommended dose of N and P fertilizers gave increase yield of cauliflower crop by 27.29% over recommended doses of NPK with a saving of 25% N and 25% P fertilizers.
Page(s): 221-227
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(2) [April 2015]

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