Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/31810
Title: Degradation of polycyclic aromatic hydrocarbons (PAHs) employing biosurfactant producing Pseudomonas aeruginosa KS3
Authors: Patowary, Kaustuvmani
Kalita, Mohan Chandra
Deka, Suresh
Keywords: Biosurfactant;Crude oil;PAHs degradation;Pseudomonas aeruginosa KS3;TPH degradation
Issue Date: Apr-2015
Publisher: NISCAIR-CSIR, India
Abstract: An efficient biosurfactant-producing/hydrocarbon-degrading native bacterial strain Pseudomonas aeruginosa KS3 (identified by partial 16S rDNA gene sequencing) was isolated from crude oil contaminated soil collected from the oil field of Lakowa in Sivasagar district of Assam, India. Experiments were conducted in the laboratory to determine the efficiency of strain KS3 to degrade polycyclic aromatic hydrocarbons (PAHs). Further, biosurfactant production was measured based on surface tension (ST) reduction of culture media. The results of the study show that this native biosurfactant producing bacterial strain has great potentiality in the degradation of total petroleum hydrocarbon (TPH) and PAHs, which have great prospects in remediation of hydrocarbon from contaminated sites. It was found that the strain KS3 could degrade 79.16% of TPH in 4 wk of incubation time. The strain also demonstrated efficient degradation of PAHs. Among the 16 major PAHs present in the crude oil sample, strain KS3 could completely degrade 8 of them. Biochemical and FTIR analyses confirmed that the produced biosurfactant was glycolipid in nature.
Page(s): 208-215
URI: http://hdl.handle.net/123456789/31810
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(2) [April 2015]

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