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Title: Comparative studies on ability of N-acetylated chitooligosaccharides to scavenge reactive oxygen species and protect DNA from oxidative damage
Authors: Salgaonkar, Neeta
Prakash, Divya
Nawani, N N
Kapadnis, B P
Keywords: Antioxidant;N-acetylated chitooligosaccharides;Comet assay;Oxidative DNA damage;Oxidative stress;Reactive oxygen species
Issue Date: Apr-2015
Publisher: NISCAIR-CSIR, India
Abstract: Antioxidants have key role in scavenging free radicals and have been considered as dietary part of health conscious population for reducing the risk of many diseases. In the present study, commercially available N-acetylated chitooligosaccharides [(GlcNAc)n; n=2, 3, 5, 6] were studied for their antioxidant ability against superoxide, hydroxyl, DPPH radicals and H2O2 by various radical scavenging assays. The N-acetylated chitooligosaccharides exhibited radical scavenging activities in the range of 49.1-63% for hydroxyl radical and 41-53.3% for superoxide radical. Their antioxidant ability was at par or better than standard antioxidants like butylated hydroxyanisole and mannitol. These N-acetylated chitooligosaccharides were also evaluated for their ability to prevent oxidative DNA damage in peripheral blood mononuclear cells exposed to H2O2. (GlcNAc)2, (GlcNAc)3, (GlcNAc)5 and (GlcNAc)6 decreased the oxidative DNA damage due to H2O2 by 59.5, 55.5, 22.9 and 4.9%, respectively. This antioxidative potential of N-acetylated chitooligosaccharides can be extended to beneficial health applications.
Page(s): 186-192
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(2) [April 2015]

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