<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Assessment of free-radical scavenging activities of mangiferin from <i style="mso-bidi-font-style:normal">Curcuma amada</i> obtained by non-conventional extraction methods: A comparative study</span>

Abstract

<span style="font-size:11.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Mangiferin (MgFn) is a naturally occurring bioactive glucosylxanthone having numerous pharmacological activities. A feasible microwave assisted extraction (MAE) technique for extraction of MgFn from <i style="mso-bidi-font-style: normal">Curcuma amada</i> (mango ginger) was developed in our laboratory. To prove the efficacy of MAE technique, it was compared with other methods like maceration extraction (ME) and ultrasonic sound assisted extraction (UAE). A high yield of MgFn was obtained by MAE (1.472 mg/g) compared to 1.017 mg/g yield by UAE and 0.837 mg/g by ME technique in acetone-water (50:50 v/v). Scanning electron microscopy (SEM) photographs of <i style="mso-bidi-font-style:normal">C</i><span style="mso-bidi-font-style:italic">.<i style="mso-bidi-font-style:normal"> amada</i> cells were taken, which were subjected to different extraction forces mentioned above. The efficacy of MgFn as a radical scavenger was studied using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), nitrate scavenging and ferric reducing power assays. The IC₅₀ value of MgFn extracted with MAE was recorded to be 26 µg/mL; showing it to be an effective radical scavenger more efficient than ascorbic acid.</span></span>