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Title: Differential proteomics approach to identify putative protective antigens of Mycobacterium tuberculosis presented during early stages of macrophage infection and their evaluation as DNA vaccines
Authors: Sharma, Shingar
Rajmani, RS
Kumar, Arun
Bhaskar, Ashima
Singh, Amit
Manivel, Venkatasamy
Tyagi, Anil K
Rao, Kanury VS
Keywords: BCG vaccine;Early antigens;MHC;Pulmonary disease;Tuberculosis (TB)
Issue Date: Jul-2015
Publisher: NISCAIR-CSIR, India
Abstract: Unsatisfactory performance of the existing BCG vaccines, especially against the adult pulmonary disease, has urged the need for an effective vaccine against tuberculosis (TB). In this study, we employed differential proteomics to obtain a list of antigens as potential vaccine candidates. Bacterial epitopes being presented at early stages on MHC class I and class II molecules of macrophages infected with Mycobacterium tuberculosis (M. tb) were identified using iTRAQ labelling and reverse phase LC-MS/MS. The putative vaccine candidates, thus identified, were tested as plasmid DNA vaccines in mice to ascertain their protective efficacy against the aerosolized M. tb challenge, based on their ability to reduce the bacterial load in the lungs of infected mice. Here, we observed that 4 out of the 17 selected antigens imparted significant protection against the challenge of M. tb. The four shortlisted antigens were further assessed in a more stringent guinea pig model, where too, they demonstrated significant protection. It concludes that combining a proteomics approach with the in vivo assessment of vaccine candidates in animal models can be valuable in identifying new potential candidates to expand the antigenic repertoire for novel vaccines against TB.
Page(s): 429-439
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.53(07) [July 2015]

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IJEB 53(7) 429-439 (Supplementary File 6).pdfSupplementary data31.09 kBAdobe PDFView/Open
IJEB 53(7) 429-439 (Supplementary File 5).pdfSupplementary data1.04 MBAdobe PDFView/Open
IJEB 53(7) 429-439 (Supplementary File 4).pdfSupplementary data112.28 kBAdobe PDFView/Open
IJEB 53(7) 429-439 (Supplementary File 3).pdfSupplementary data1.06 MBAdobe PDFView/Open
IJEB 53(7) 429-439 (Supplementary File 2).pdfSupplementary data371.53 kBAdobe PDFView/Open
IJEB 53(7) 429-439 (Supplementary File 1).pdfSupplementary data248.91 kBAdobe PDFView/Open
IJEB 53(7) 429-439 (Supplementary Data Captions).pdfLegends for supplementary files52.29 kBAdobe PDFView/Open
IJEB 53(12) (Erratum).pdfErratum288.94 kBAdobe PDFView/Open
IJEB 53(7) 429-439.pdfMain article730.35 kBAdobe PDFView/Open

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