Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/3160
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dc.contributor.authorBajaj, Bijender K-
dc.contributor.authorPangotra, Himani-
dc.contributor.authorWani, Masood A-
dc.contributor.authorSharma, Ajay-
dc.contributor.authorSharma, Priyanka-
dc.date.accessioned2009-02-18T10:25:14Z-
dc.date.available2009-02-18T10:25:14Z-
dc.date.issued2009-03-
dc.identifier.issn0022-4456-
dc.identifier.urihttp://hdl.handle.net/123456789/3160-
dc.description242-247en_US
dc.description.abstractThis study presents isolation and characterization of β-glucosidase (BG) from a bacterial isolate. Enzyme showed maximum activity at 50°C (2200 IU/l), however, relatively good activity (1600-1900 IU/l) was maintained even at higher temperature (60-80°C). Enzyme was thoroughly stable at 50-80°C for 30 min to 1 h and retained activity (75-100%), indicating its substantial thermo-tolerant nature. Maximum activity of BG was found at pH 6 (2200 IU/l), but significantly good activity was also observed at pH 5 (1800 IU/l), 7 (2000 IU/l), 8 (1900 IU/l) and 9 (1500 IU/l). Pb²⁺ caused considerable increase in enzyme activity (47%), while Zn²⁺ caused a little reduction (7%). SDS-PAGE showed a single band of BG (mol wt, 24 kDa).en_US
dc.language.isoen_USen_US
dc.publisherCSIRen_US
dc.sourceJSIR Vol.68(3) [March 2009]en_US
dc.subjectAcid/alkali toleranten_US
dc.subjectCharacterizationen_US
dc.subjectβ-Glucosidaseen_US
dc.subjectPurificationen_US
dc.subjectThermo-toleranten_US
dc.titleCharacterization of thermo-tolerant and acid/alkali tolerant β-glucosidase from bacterial isolate M+en_US
dc.typeArticleen_US
Appears in Collections:JSIR Vol.68(03) [March 2009]

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