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Title: Characterization of thermo-tolerant and acid/alkali tolerant β-glucosidase from bacterial isolate M+
Authors: Bajaj, Bijender K
Pangotra, Himani
Wani, Masood A
Sharma, Ajay
Sharma, Priyanka
Keywords: Acid/alkali tolerant;Characterization;β-Glucosidase;Purification;Thermo-tolerant
Issue Date: Mar-2009
Publisher: CSIR
Abstract: This study presents isolation and characterization of β-glucosidase (BG) from a bacterial isolate. Enzyme showed maximum activity at 50°C (2200 IU/l), however, relatively good activity (1600-1900 IU/l) was maintained even at higher temperature (60-80°C). Enzyme was thoroughly stable at 50-80°C for 30 min to 1 h and retained activity (75-100%), indicating its substantial thermo-tolerant nature. Maximum activity of BG was found at pH 6 (2200 IU/l), but significantly good activity was also observed at pH 5 (1800 IU/l), 7 (2000 IU/l), 8 (1900 IU/l) and 9 (1500 IU/l). Pb²⁺ caused considerable increase in enzyme activity (47%), while Zn²⁺ caused a little reduction (7%). SDS-PAGE showed a single band of BG (mol wt, 24 kDa).
Page(s): 242-247
ISSN: 0022-4456
Appears in Collections:JSIR Vol.68(03) [March 2009]

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