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|Title:||Optimization of method to alleviate in vitro shoot tip necrosis in Trichosanthes dioica Roxb.|
Shukla, A K
|Keywords:||Calcium chloride;Multiplication index;Trichosanthese dioica;Shoots tip necrosis|
|Abstract:||Shoot tip necrosis (STN) has been observed to be the major limiting factor for in vitro regeneration of Trichosanthes dioica Roxb. The intensity of necrosis at shoot multiplication stage was relatively higher (82%) when compared with rooting stage (64%). The necrosis appeared with the yellowing of apical leaves after 1 wk of first subculture (14 d) and progressively increased resulting in leaf burn and death of more than 80% of cultures within 5 wk. Among various methods, viz., subculture intervals, manipulation in media salt strength, levels of plant growth regulators, sucrose, calcium and boron, tested to control STN, only calcium significantly alleviated the intensity of necrosis. Transfer of affected cultures to Murashige and Skoog medium supplemented with 0.68 mM calcium chloride reduced the STN intensity by about 90% both at shoot multiplication and rooting stages. Additionally, calcium supplemented medium gave maximum multiplication index and axial and lateral shoot length. Apart from calcium, subculture interval and boron also reduced the incidence of STN though at low level. Subculture at 1 wk interval had 52% STN incidence compared to those subcultured at 2 wk (85%) and 3 wk intervals (100%), whereas boron (0.64 µM) could reduce the incidence up to 24%.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.14(1) [January 2015]|
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