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|Title:||Genetic diversity of sulphur oxidizing bacteria from different ecosystems|
|Keywords:||RuBisCO;Sox B gene;Sulfate production;Sulphur oxidizing bacteria|
|Abstract:||Sulphur is one of the fourth major nutrients after N, P, and K for oil crops and pulses. To explore the possibility of meeting the sulphur nutrition of groundnut crop through bioinoculant, in the present study, a total of 24 isolates of sulphur oxidizing bacteria (SOB) obtained from different ecosystems were screened. Among the screened isolates, VSS4 isolate produced 72 mg 100 mL-1 sulphate from elemental sulphur. Species diversity, phylogenetic affiliations and environmental occurrence patterns of SOB were investigated by amplified ribosomal DNA restriction analysis (ARDRA). Finally, based upon the suphate production, pH reduction and other biochemical studies, four isolates, viz.,VSS4, VSM8, VSB3 and VST3, were selected for the study of genetic diversity between different ecosystems by using 16S ribosomal RNA (rRNA) gene sequences and phylogenetic analysis. SOB isolates VSS4, VSM8, VSB3 and VST3 were identified as Pseudomonas sp., Burkholderia sp., Exiguobacterium sp., and Bacillus sp., respectively. For the conformation of their chemoautotrophic nature, the selected isolates were screened with the primers of cbbL (form I), cbbM (form II) genes of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO). All the four selected isolates were chemoautotrophic in nature as they showed the presence of cbbL (form I) gene.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.14(1) [January 2015]|
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