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http://nopr.niscair.res.in/handle/123456789/31235
Title: | Anticancer and anti-inflammatory activities of some dietary cucurbits |
Authors: | Sharma, Dhara Rawat, Indu Goel, HC |
Keywords: | Annexin V assay;Apoptosis;Cancer;Cell viability;Caspase-3 assay;Cucurbita pepo;Cucurbitacin-B;IL-8 cytokines;Inflammation;Lagenaria siceraria;Luffa cylindrica;Lymphocytes;Tumor cells |
Issue Date: | Apr-2015 |
Publisher: | NISCAIR-CSIR, India |
Abstract: | In this study, we investigated few dietary cucurbits for anticancer activity by monitoring cytotoxic (MTT and LDH assays), apoptotic (caspase-3 and annexin-V assays), and also their anti-inflammatory effects by IL-8 cytokine assay. Aqua-alcoholic (50:50) whole extracts of cucurbits [Lagenaria siceraria (Ls), Luffa cylindrica (Lc) and Cucurbita pepo (Cp)] were evaluated in colon cancer cells (HT-29 and HCT-15) and were compared with isolated biomolecule, cucurbitacin-B (Cbit-B). MTT and LDH assays revealed that the cucurbit extracts and Cbit-B, in a concentration dependent manner, decreased the viability of HT-29 and HCT-15 cells substantially. The viability of lymphocytes was, however, only marginally decreased, yielding a potential advantage over the tumor cells. Caspase-3 assay revealed maximum apoptosis with Ls while annexin V assay demonstrated maximum efficacy of Lc in this context. These cucurbits have also shown decreased secretion of IL-8, thereby revealing their anti-inflammatory capability. The results have demonstrated the therapeutic potential of dietary cucurbits in inhibiting cancer and inflammatory cytokine. |
Page(s): | 216-221 |
URI: | http://hdl.handle.net/123456789/31235 |
ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
Appears in Collections: | IJEB Vol.53(04) [April 2015] |
Files in This Item:
File | Description | Size | Format | |
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IJEB 53(4) 216-221.pdf | 187.48 kB | Adobe PDF | View/Open |
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