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|Title:||Covalent immobilization of lipase onto onion membrane affixed on plastic surface : Kinetic properties and application in milk fat hydrolysis|
Pundir, C S
|Series/Report no.:||Int. Cl.⁸ C12N9/20, 11/08|
|Abstract:||Lipase from porcine pancreas was immobilized covalently onto onion membrane, affixed on plastic surface through non-reactive fixative, with a conjugation yield of 0.174 mg/cm² and 63.6 % retention of initial activity of free enzyme. The enzyme showed no change in optimum pH 7.5 but slight change in incubation temperature from 35 to 40ºC and time of linearity from 15 to 30 min after immobilization. The utility of membrane bound lipase to prepare fat free milk without degradation of other essential components and formation of unwanted by-products was tested employing commercial kit for TG determination. Thus, the use of immobilized lipase in hydrolysis of milk fats increases the digestion process with out consuming them. The immobilized enzyme was reused 100 times without considerable loss of activity, when stored at 4ºC for a period of 2 months.|
|Appears in Collections:||IJBT Vol.06(4) [October 2007]|
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