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|Title:||Molecular identification of Proutista moesta as the vector and the phylogenetic analysis of KWD phytoplasma|
|Authors:||Edwin, Boby T|
|Keywords:||Proutista moesta;Kerala wilt disease;phytoplasma;Universal primers;650bp|
|IPC Code:||Int. Cl.⁸ C12N15/09, 15/29|
|Abstract:||The purified genomic DNA from the tissues of Proutista moesta and Stephanitis typica, considered as possible vectors of Kerala wilt disease (KWD) of coconut palm, was subjected to PCR assay using the primer pairs P1/P6, P1/P7 and P4/P7. In case of P. moesta, the amplified products resolved a prominent band of 650 bp for the universal primer pair P4/P7; however, no bands were noticed for the primer pairs P1/P6 and P1/P7. In case of S. typica no bands were noticed for all sets of primers. Since P4/P7 amplifies the 16S-23S intergenic spacer region of 16SrRNA gene, the 650 bp product from P. moesta indicates the presence of phytoplasma DNA. The restriction enzyme analysis of the 650 bp product, using the enzymes AluI, BclI, HindIII and RsaI, further supports the phytoplasmic nature of DNA. The presence of 650 bp product in the genomic DNA of P. moesta shows the insect being a vector of KWD phytoplasma. The sequential similarity of 650 bp of both KWD phytoplasma and the insect phytoplasma again supports the transmission of phytoplasma through the vector P. moesta. From the cladogram, it is obvious that the KWD phytoplasma is evolutionarily closest to the phytoplasma causing coconut lethal yellowing of Mexican palms within the group 16SrIV.The present study is the fist confirmed record of P. moesta as the vector of KWD by detecting KWD phytoplasma in the insect tissues by PCR based methods.|
|Appears in Collections:||IJBT Vol.06(4) [October 2007]|
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