NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.47 [2009] >
IJEB Vol.47(02) [February 2009] >


Title: High frequency plantlet regeneration from rhizomatous buds in Mantisia spathulata Schult. and Mantisia wengeri Fischer and analysis of genetic uniformity using RAPD markers
Authors: Bhowmik, Sudipta Shekhar D
Kumaria, Suman
Rao, S R
Tandon, Pramod
Keywords: Mantisia spathulata
Mantisia wengeri
Plant regeneration
RAPD analysis
Issue Date: Feb-2009
Publisher: CSIR
Abstract: A protocol has been devised for enhanced in vitro regeneration of critically endangered Mantisia spathulata Schult. and Mantisia wengeri Fischer. Highest Bud Forming Capacity (BFC) of 6.10±0.55 with an average of 19.93±3.19 roots was obtained for M. spathulata within 5-6 weeks in Murashige and Skoogs (MS) medium supplemented with a combination of 10.0 μM of N⁶-benzyladenine (BA) and 2.5μM of ⍺-naphtalene acetic acid (NAA). For M. wengeri, BFC of 7.82±0.73 and 20.86±1.65 roots was achieved in MS media supplemented with a combination of 5.0μM BA and 2.5μM of NAA RAPD markers were used to evaluate the genetic stability of in vitro raised hardened plantlets. Similarity coefficient among the regenerated plants ranged between 0.85-0.98 for M. spathulata and 0.83-0.98 for M. wengeri. Maximum of 88 and 90% genetic similarity were obtained between in vitro raised hardened plantlets and mother stock of M. spathulata and M. wengeri, respectively through RAPD analysis. The hardened plantlets after RAPD analysis on being transferred to soil of experimental garden showed no marked phenotypic variations in vegetative or floral characteristics.
Page(s): 140-146
ISSN: 0019-5189
Source:IJEB Vol.47(02) [February 2009]

Files in This Item:

File Description SizeFormat
IJEB 47(2) 140-146.pdf517.3 kBAdobe PDFView/Open
 Current Page Visits: 521 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 603779 since 06-Feb-2009  Last updated on 21-Oct-2014Webmaster: nopr@niscair.res.in