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Title: RAPD markers for identifying oil palm (Elaeis guineensis Jacq.) parental varieties (dura & pisifera) and the hybrid tenera
Authors: Sathish, D K
Mohankumar, C
Keywords: Elaeis guineensis
hybrid
parental varieties
random primers
RAPD
Issue Date: Jul-2007
Publisher: CSIR
IPC CodeInt. Cl.⁸ C12N 15/10
Abstract: Random amplification of polymorphic DNA (RAPD) analysis was done by arbitrary primers for determining the DNA polymorphism among the oil palm (Elaeis guineensis) varieties dura, pisifera and tenera, and monitoring the specificity of the primers for identifying each genotype. The three varieties were evaluated using thirty, 10-mer primers. Of the 30 primers, 26 yielded significant polymorphic DNA bands. A total of 185 bands were determined (300-1500 bp), of which 59 were representing dura, 65 for pisifera and 61 bands for tenera. The dendrogram obtained, using UPGMA, grouped all the three varieties into two distinct clusters. In one cluster, the shelled ones (dura and tenera) were grouped, while the other comprised the shell-less one (pisifera). The data also provide sufficient evidence for identifying each variety, dura, pisifera and tenera separately as well as the parentals dura and pisifera together. This is the first report of DNA based polymorphism assay to assess the level of variability in oil palm varieties.
Page(s): 354-358
ISSN: 0972-5849
Source: IJBT Vol.06(3) [July 2007]

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