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Title: Genetic analysis of CMS, restorer, hybrid and open-pollinated genotypes of Indian pearl millet [Pennisetum glaucum (L.) R. Br.] using ISSR markers
Authors: Yadav, Satish
Jain, Sunita
Jain, Veena
Jain, Rajinder K
Keywords: genetic diversity;genotyping;ISSR;pearl millet;Pennisetum glaucum;CMS;restorer;OPV
Issue Date: Jul-2007
Publisher: CSIR
IPC Code: Int. Cl.⁸ C12N15/10
Abstract: Genetic diversity and relationships among 20 Indian pearl millet [Pennisetum glaucum (L.) R. Br.] genotypes representing the commercially important hybrids, cytoplasmic male sterile (CMS) lines, restorer (res) lines and open pollinated varieties (opv) were assessed using inter simple sequence repeat (ISSR) markers. A total of 349 reproducible bands (73 monomorphic, 276 polymorphic) were detected using 30 UBC#9 ISSR primers with polymorphism percentage of 79.1%. Fortyfive of the polymorphic bands were in fact the unique bands that were present or absent exclusively in only one of the 20 genotypes. A set of two or any five ISSR-PCR assays were sufficient to clearly distinguish among the 20 genotypes. The salient features of ISSR marker data analyzed using clustering algorithms (NTSYS-Pc and Win Boot) and principal component analysis (two-dimensional, NTSYS-pc) are as given below: (i) all the four restorer lines, which have been widely used for hybrid production, were clustered in a single group, which is indicative of their narrow genetic base, (ii) the seven CMS lines, which are basically bred from Tift23A or Tift23A derived lines after crosses/backcrosses with some downy mildew resistance sources, displayed low to moderate levels of polymorphism, (iii) the three OPVs were quite diverse with HC-20 being more distant to HC-10 and HC-4, and (iv) hybrids such as HHB-60 and HHB-67, with same restorer parentage exhibited greater relatedness. The high resolution and reproducibility of ISSR-PCR assay shown in this study shall provide a simple, rapid and cost-effective system for the characterization of huge collection of germplasm and testing of purity of genetic stocks/hybrids. The study also demonstrates the need for selecting/generating genetically diverse restorer as well as CMS lines for the breeding of genetically diverse hybrids in pearl millet.
Page(s): 340-348
ISSN: 0972-5849
Appears in Collections: IJBT Vol.06(3) [July 2007]

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