NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(1) [January 2007] >


Title: Assessment of genetic diversity among some Indian wheat cultivars using random amplified polymorphic DNA (RAPD) markers
Authors: Grewal, Sapna
Kharb, Pushpa
Malik, Rekha
Jain, Sunita
Jain, R K
Keywords: wheat
RAPD
genetic diversity
PCR
varietal identification
Issue Date: Jan-2007
Publisher: CSIR
IPC CodeInt, Cl.⁸: C12 N15/10
Abstract: Genetic relationship study was performed with RAPD primers among 20 Indian wheat accessions (17 hexaploids, 2 tetraploids and 1 diploid). A total of 372 bands were observed using 25 RAPD primers, of which 323 (86.8%) were polymorphic. The total number of amplification products per primer ranged from 5 (OPO-02) to 30 (OPH-01) with an average of 14.8±1.15 bands per primer. The size of PCR products ranged from 162 bp to 2529 bp. A significant corelation (0.933, p<0.01) was observed between the total number of bands and the number of polymorphic bands. Sixteen primers amplified 30 unique bands for specific genotypes that would prove to be highly useful in identification of genotype and designing future breeding strategy. The similarity coefficient values ranged from 0.52 to 0.82, indicating high genetic variability among the wheat genotypes. The 20 wheat genotypes were grouped into two major clusters. Cluster I had the diploid genotype T. urartu, tetraploid variety WH 896 and hexaploid variety PBW 175. Rest of the 16 hexaploid varieties and a tetraploid variety (Khapli) were placed in the other major cluster.
Page(s): 18-23
ISSN: 0972-5849
Source: IJBT Vol.06(1) [January 2007]

Files in This Item:

File Description SizeFormat
IJBT 6(1) 18-23.pdf770.91 kBAdobe PDFView/Open
 Current Page Visits: 595 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 576010 since 06-Feb-2009  Last updated on 26-Aug-2014Webmaster: nopr@niscair.res.in