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|Title:||Partial purification and characterization of amylase and starch from Elephant foot yam [Amorphophallus campanulatus (Roxb.) Blume]|
Gowda, T Veerabasappa
|Keywords:||Amylase;Endogenous starch;Isoforms;Kinetic characterization;Zymogram|
|Abstract:||Amylase (EC 18.104.22.168) and starch from Amorphophallus campanulatus (Roxb.) Blume (Elephant foot yam) were partially purified. Kinetic characterization of yam amylase at its optimum conditions of activity was carried out. Its activity profile was compared with that of salivary amylase upon yam and commercial potato starch as substrates. A differential behaviour in the activity of yam amylase compared to salivary amylase was observed. Presence of isoforms of amylase in yam was detected. The partially degraded yam starch appeared to be better substrate compared to native starch. Ca2+ ions stimulated the yam amylase activity upon endogenous starch than Na+ and K+ ions.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.13(4) [October 2014]|
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