Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/30485
Title: Comparative study of cytokinin production isolated from bacteria and shoot induction
Authors: Sabat, Sasmita
Murthy, V Krishna
Shantha, S L
Kushnoor, Divyashree
Agarwal, Garima
Thomas, Jennifer
Devaraj, Surabhi
Keywords: Bacillus subtilis;Cytokinin;Escherichia coli;Phillodendron xanadu;Rhizobium sp.;Shoot induction
Issue Date: Oct-2014
Publisher: NISCAIR-CSIR, India
Abstract: Rhizobium sp., isolated from Mimosa pudica, Bacillus subtilis BC1 and Escherichia coli K12 were investigated for their ability to produce plant growth hormones in a given production media (PM1 & PM2). Thin layer chromatography and high pressure liquid chromatography were performed to analyse the amount of cytokinin produced by these microorganisms. E. coli K12 produced the highest amount (0.186 mg/L) of cytokinin in PM2 medium, while B. subtilis BC1 gave the least production amount (0.0075 mg/L) in the same medium. The microbially synthesized cytokinin was then incorporated onto the solidified MS medium for the shoot development in Phillodendron xanadu, an ornamental plant. The cytokinin produced from E. coli K12 gave more number of plantlets with thick shoot and lustrous leaves having 3.0 cm length for medium with charcoal (PM2); while comparatively less number of plantlets having 1.5 cm length for medium without charcoal. The cytokinin from B. subtilis BC1 and Rhizobium sp. gave comparatively more shoot number and more plant height on medium with charcoal in PM1 and PM2 respectively.
Page(s): 544-546
URI: http://hdl.handle.net/123456789/30485
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.13(4) [October 2014]

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