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|Title:||A simple and rapid DNA extraction protocol for filamentous fungi efficient for molecular studies|
|Keywords:||DNA isolation;Filamentous fungi;PCR-RFLP;RAPD;18S rRNA|
|Abstract:||A simple and rapid protocol for extracting high-quality DNA from filamentous fungi was studied. The method involved disruption of fungal cells by employing glass bead method, followed by inactivation of proteins using CTAB/proteinase K. The DNA yield from fungal isolates varied from 310-1879 µg g-1 dry mycelium and a clear intact DNA band was observed upon agarose gel electrophoresis. Absorbency ratios (A260/A280) for DNA ranged 1.7-1.9, which indicated minimal presence of contaminating metabolites. PCR analysis like 18S rRNA gene amplification, random amplified polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) showed that DNA was compatible for downstream applications. This method can be applied to extract genomic DNA of filamentous fungi from different environmental sources.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.13(4) [October 2014]|
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