Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/30483
Title: A simple and rapid DNA extraction protocol for filamentous fungi efficient for molecular studies
Authors: Gontia-Mishra, Iti
Tripathi, Niraj
Tiwari, Sharad
Keywords: DNA isolation;Filamentous fungi;PCR-RFLP;RAPD;18S rRNA
Issue Date: Oct-2014
Publisher: NISCAIR-CSIR, India
Abstract: A simple and rapid protocol for extracting high-quality DNA from filamentous fungi was studied. The method involved disruption of fungal cells by employing glass bead method, followed by inactivation of proteins using CTAB/proteinase K. The DNA yield from fungal isolates varied from 310-1879 µg g-1 dry mycelium and a clear intact DNA band was observed upon agarose gel electrophoresis. Absorbency ratios (A260/A280) for DNA ranged 1.7-1.9, which indicated minimal presence of contaminating metabolites. PCR analysis like 18S rRNA gene amplification, random amplified polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) showed that DNA was compatible for downstream applications. This method can be applied to extract genomic DNA of filamentous fungi from different environmental sources.
Page(s): 536-539
URI: http://hdl.handle.net/123456789/30483
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.13(4) [October 2014]

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