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Title: Molecular characterization of MHC-DRB gene exon 2 in riverine buffalo (Bubalus bubalis)
Authors: Naskar, Soumen
Deb, Sitangsu M
Niranjan, Saket K
Sakaram, Durgam
Sharma, Deepak
Kumar, Subodh
Sharma, Arjava
Keywords: Buffalo;DRB;MHC;PCR-RFLP;Phylogeny
Issue Date: Oct-2014
Publisher: NISCAIR-CSIR, India
Abstract: In the ┬ápresent study, expressed DRB gene of buffalo major histocompatibility complex (Bubu) was characterized. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of Bubu-DRB second exon region using TaqI and HaeIII restriction enzymes revealed one and three restriction patterns, respectively. Comparative analysis of two different HaeIII variants revealed large number of amino acids substitutions at peptide binding site (PBS) of the molecule. The variants showed low divergence with cattle (BoLA) DRB3 alleles in general. Phylogenetically, Bubu-DRB clone b and e were found to be closer to Bubu-DRB*0501 and DRB*0401 alleles, respectively. High genetic variation at PBS region of Bubu-DRB exon 2 might contribute positively to antigen repertoire properties during infection.
Page(s): 532-535
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.13(4) [October 2014]

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