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Title: Effect of different factors on in vitro axillary shoot proliferation and plant regeneration of Cinnamomum tamala Nees: A spice yielding plant
Authors: Deb, Chitta Ranjan
Deb, Madhabi S
Jamir, N S
Keywords: Axillary bud breaking;Cinnamomum tamala;Media strength;Micropropagation;Nodal explants
Issue Date: Oct-2014
Publisher: NISCAIR-CSIR, India
Abstract: In the present study, a successful attempt was made to establish the aseptic culture of Cinnamomum tamala Nees from nodal explants and to evaluate the factors influencing in vitro morphogenesis. About 45.6% nodal explants collected during July established successfully on full strength MS medium fortified with sucrose (3%, w/v), polyvinyl pyrollidone (PVP; 100 mg L-1) and benzyl adenine (BA; 9 µM), where as many as 5.4 meristematic loci were invoked after 5-6 wk of culture initiation. The shoot buds developed on initiation medium converted into microshoots on transferring to MS medium conjunct with sucrose (3%) and kinetin (Kn; 3 µM). The microshoots were rooted by culture on MS medium fortified with sucrose (3%) and α-naphthalene acetic acid (NAA; 3 µM), where as many as 10 roots developed per microshoot. The rooted plantlets were transferred to potting mix containing soil, sand and decayed wood powder at 1:1:1 ratio and maintained in the polyhouse at ca 75% shading of light for 2 months. The acclimatized plantlets were transferred to the nature and registered 70% survival after 2 months of transfer.
Page(s): 520-526
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.13(4) [October 2014]

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