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Title: A repeatable protocol for in vitro micropropagation of mulberry variety S₅₄
Authors: Kavyashree, R
Keywords: apical bud
ex vitro rooting
multiple shoots
survival frequency
Issue Date: Jul-2007
Publisher: CSIR
Series/Report no.: Int. Cl.⁸ A01H4/00, 5/04
Abstract: Apical buds of mulberry (Morus indica L.) variety S₅₄ were cultured on LSBM supplemented with different concentrations of BAP and TIBA to study their in vitro micropropagation potential. It was found that LSBM fortified with 8.88 µM BAP in combination with 2 µM TIBA was the most suitable medium for initiation (94% response) and multiplication of shoots (10.6) when compared to other concentrations tried. The well developed, elongated, healthy multiple shoots were subjected to ex vitro rooting technique to obtain complete plantlets. The statistical analysis of the data revealed significant difference between and within the treatments with regard to multiple shoots and root formation. The well developed axenic plants with similar genetic makeup were successfully established in the field with 90% survival frequency through sequential hardening process.
Description: 385-388
ISSN: 0972-5849
Appears in Collections: IJBT Vol.06(3) [July 2007]

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