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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(3) [July 2007] >
| Title: | A repeatable protocol for in vitro micropropagation of mulberry variety S₅₄ |
| Authors: | Kavyashree, R |
| Keywords: | apical bud
ex vitro rooting
micropropagation
multiple shoots
survival frequency |
| Issue Date: | Jul-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.⁸ A01H4/00, 5/04 |
| Abstract: | Apical buds of mulberry (Morus indica L.) variety S₅₄ were cultured on LSBM supplemented with different concentrations of BAP and TIBA to study their in vitro micropropagation potential. It was found that LSBM fortified with 8.88 µM BAP in combination with 2 µM TIBA was the most suitable medium for initiation (94% response) and multiplication of shoots (10.6) when compared to other concentrations tried. The well developed, elongated, healthy multiple shoots were subjected to ex vitro rooting technique to obtain complete plantlets. The statistical analysis of the data revealed significant difference between and within the treatments with regard to multiple shoots and root formation. The well developed axenic plants with similar genetic makeup were successfully established in the field with 90% survival frequency through sequential hardening process. |
| Page(s): | 385-388 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.06(3) [July 2007]
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