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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(2) [April 2007] >
| Title: | Molecular markers for screening salinity response in Sorghum |
| Authors: | Rao, M V Subba
Kumari, P Kusuma
Manga, V
Mani, N Sarada |
| Keywords: | RAPD
RFLP
salinity tolerance
Sorghum |
| Issue Date: | Apr-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.⁸ C12N 15/10 |
| Abstract: | Genomic DNAs from two salinity tolerant (IS 23190-Zm 90 & IS 23217-Zm 138), one moderately tolerant (IS 3566) and one sensitive(IS 213253-Zm 321) accessions of Sorghum were subjected to RFLP and RAPD analyses. A combination of digestion with Dra I and probing with BADH 1 distinguished the sensitive from the tolerant and moderately tolerant accessions in the RFLP patterns. A primer having CGGCTAGGT sequence provided distinct RAPD pattern for the tolerants (presence of specific amplified products a, b & c), moderately tolerant (presence of only c) and sensitive (lacking a, b & c) genotypes. |
| Page(s): | 271-273 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.06(2) [April 2007]
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