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Title: Molecular markers for screening salinity response in Sorghum
Authors: Rao, M V Subba
Kumari, P Kusuma
Manga, V
Mani, N Sarada
Keywords: RAPD;RFLP;salinity tolerance;Sorghum
Issue Date: Apr-2007
Publisher: CSIR
IPC Code: Int. Cl.⁸ C12N 15/10
Abstract: Genomic DNAs from two salinity tolerant (IS 23190-Zm 90 & IS 23217-Zm 138), one moderately tolerant (IS 3566) and one sensitive(IS 213253-Zm 321) accessions of Sorghum were subjected to RFLP and RAPD analyses. A combination of digestion with Dra I and probing with BADH 1 distinguished the sensitive from the tolerant and moderately tolerant accessions in the RFLP patterns. A primer having CGGCTAGGT sequence provided distinct RAPD pattern for the tolerants (presence of specific amplified products a, b & c), moderately tolerant (presence of only c) and sensitive (lacking a, b & c) genotypes.
Page(s): 271-273
ISSN: 0972-5849
Appears in Collections: IJBT Vol.06(2) [April 2007]

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