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Title: Efficient Pseudomonas putida for degradation of p-nitrophenol
Authors: Kulkarni, Meenal
Chaudhari, Ambalal
Keywords: p-nitrophenol;Biodegradation;Pseudomonas putida;Hydroquinone;Ring fission
Issue Date: Jul-2006
Publisher: NISCAIR-CSIR, India
Abstract: Exploration of Pseudomonas putida for controlling environmental (p-nitrophenol) PNP pollution revealed that 0.5 OD inoculum at 600 nm degraded 100 ppm of PNP completely under the ambient conditions within 24 h as established by UV-visible spectrophotometric and TLC analysis of treated broth and confirmed the same by HPLC analysis. An attempt to elucidate the mechanism of PNP degradation indicated that (i) it was oxidative with a stoichiometric release of nitrite, (ii) PNP was converted to hydroquinone, which accumulated in the presence of 2,2' -dipyridyl, (iii) hydroquinone was the first product of ring oxygenation and nitrite removal, as confirmed by TLC and HPLC analysis and (iv) concomitant formation of a keto acid by ring fission, which was confirmed by Rothera's test.  
Page(s): 411-415
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(3) [July 2006]

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