NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(2) [April 2007] >

Title: Immobilization of tannase from Rhizopus oryzae and its efficiency to produce gallic acid from tannin rich agro-residues
Authors: Hota, Swetansu K
Dutta, Jayati Ray
Banerjee, Rintu
Keywords: immobilization
Rhizopus oryzae
sal seed
Issue Date: Apr-2007
Publisher: CSIR
IPC CodeInt. Cl.⁸ A01N63/04; C12N9/20; C12N11/04
Abstract: Different tannin rich agro-residues, like sal seed (21% tannin), fruit of myrobalan (37.6% tannin) and tea-leaf (14.1% tannin) were selected for enzymatic conversion of their tannin content to gallic acid. These tannin rich substrates were used as carbon source in Czapek-dox medium for the production of tannase (E.C., tannin acyl hydrolase) from Rhizopus oryzae (RO IIT RB-13, NRRL-21498). The maximum enzyme production of 17.7 U/mL was obtained in sal seed powder incubated for 48 h at 30ºC. The enzymatic conversion of these agro-residues was carried out using immobilized tannase. Substrate concentrations of 8% sal seed, 7% myrobalan and 6% tea leaf (w/v) were found to be the optimum for maximum bioconversion. The maximum bioconversion (90 and 87%, respectively) was achieved with sal seed and tea leaf as substrate at 40ºC and initial pH 4.5. In case of myrobalan, the maximum bioconversion was 90.2% at 50ºC and initial pH 5.0. Moreover, optimization of the pH and temperature largely reduced the incubation time to 36 h. The immobilized tannase was stable for 7 cycles. The kinetic properties of immobilized enzyme revealed that there was a decrease in maximal reaction velocity (Vmax) and increase in Michaelis constant (Km) when compared to its free native counterpart.
Page(s): 200-204
ISSN: 0972-5849
Source: IJBT Vol.06(2) [April 2007]

Files in This Item:

File Description SizeFormat
IJBT 6(2) 200-204.pdf233.2 kBAdobe PDFView/Open
 Current Page Visits: 271 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 169518 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: