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Title: Phytochemical and antimicrobial activities of Himalayan Cordyceps sinensis (Berk.) Sacc.
Authors: Mamta
Mehrotra, Shubhi
Kirar, Vandana
Vats, Praveen
Nandi, Shoma Paul
Negi, PS
Misra, Kshipra
Keywords: Antimicrobial activity;Antioxidant;Bioactive nucleoside;Bioactive nucleoside;Herbal
Issue Date: Jan-2015
Publisher: NISCAIR-CSIR, India
Abstract: This study evaluated the phytochemical and antimicrobial activities and also quantified bioactive nucleoside using high performance thin layer chromatography (HPTLC) of five extracts of Indian Himalayan Cordyceps sinensis prepared with different solvents employing accelerated solvent extraction (ASE) technique. The phytochemical potential of these extracts was quantified in terms of total phenolic and total flavonoid content while antioxidant activities were determined by 1,1-diphenyl-2-pycryl-hydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric-reducing antioxidant power (FRAP) assays. Total reducing power (TRP) was determined by converting iron (III) into iron (II) reduction assay. CS50%Alc (15.1+ 0.67mg/g of dry extract) and CS100%Alc (19.3 + 0.33mg/g of dry extract) showed highest phenolic and flavonoid content, respectively while CSAq extract showed maximum antioxidant activity and the highest concentration of the three nucleosides (adenine 12.8±0.49 mg/g, adenosine 0.36±0.28 mg/g and uracil 0.14±0.36 mg/g of dry extract) determined by HPTLC. The evaluation of extracts for antimicrobial activity against gram-negative and gram-positive bacterial strains showed CS25%Alc, CS75%Alc and CS100%Alc extract to be more effective against E. coli, P. aerugenosa and B. subtilis giving 9, 7 and 6.5 mm of zone of inhibition (ZOI) in 93.75, 93.75 and 45 µg concentration, respectively, whereas CSAq extract showed minimal inhibition against these.
Page(s): 36-43
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.53(01) [January 2015]

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