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|Title:||Plantlet regeneration of Pinus kesiya Royle ex Gord. from mature embryos|
|Keywords:||growth characteristics;micropropagation;shoot buds induction;zygotic embryos;Pinus kesiya|
|IPC Code:||Int. Cl.⁸ A01H4/00, 7/00|
|Abstract:||A high frequency (90.5%) of shoot bud induction was observed in mature zygotic embryos of Pinus kesiya Royle ex Gord. that were pre-cultured in Quoirin and Lepoivre (LP) medium containing 23.15 μM Kn for 4 wk and then transferred to growth regulator-free medium. Multiplication and elongation of the shoot buds resulted in 1/2 LP medium containing 0.5 μM IBA and 4.5 μM BAP or Kn. Rooting was 72.3% in isolated shoots that were treated with 53.76 μMNAA for 24 h and then cultured on water-agar medium. Plantlets were hardened and successfully established on soil collected from pine forests with 70% survival. Biomass of the micropropagated plants was 3.2 times higher than seed-derived plants.|
|Appears in Collections:|| IJBT Vol.06(2) [April 2007]|
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