NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(2) [April 2007] >

Title: Plantlet regeneration of Pinus kesiya Royle ex Gord. from mature embryos
Authors: Tandon, Pramod
Kumaria, Suman
Choudhury, Hiranjit
Keywords: growth characteristics
shoot buds induction
zygotic embryos
Pinus kesiya
Issue Date: Apr-2007
Publisher: CSIR
IPC CodeInt. Cl.⁸ A01H4/00, 7/00
Abstract: A high frequency (90.5%) of shoot bud induction was observed in mature zygotic embryos of Pinus kesiya Royle ex Gord. that were pre-cultured in Quoirin and Lepoivre (LP) medium containing 23.15 μM Kn for 4 wk and then transferred to growth regulator-free medium. Multiplication and elongation of the shoot buds resulted in 1/2 LP medium containing 0.5 μM IBA and 4.5 μM BAP or Kn. Rooting was 72.3% in isolated shoots that were treated with 53.76 μMNAA for 24 h and then cultured on water-agar medium. Plantlets were hardened and successfully established on soil collected from pine forests with 70% survival. Biomass of the micropropagated plants was 3.2 times higher than seed-derived plants.
Page(s): 262-266
ISSN: 0972-5849
Source: IJBT Vol.06(2) [April 2007]

Files in This Item:

File Description SizeFormat
IJBT 6(2) 262-266.pdf2.26 MBAdobe PDFView/Open
 Current Page Visits: 99 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 168696 since 01-Sep-2015  Last updated on 29-Jun-2016Webmaster: