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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.06 [2007] >
IJBT Vol.06(2) [April 2007] >
| Title: | Plantlet regeneration of Pinus kesiya Royle ex Gord. from mature embryos |
| Authors: | Tandon, Pramod
Kumaria, Suman
Choudhury, Hiranjit |
| Keywords: | growth characteristics
micropropagation
shoot buds induction
zygotic embryos
Pinus kesiya |
| Issue Date: | Apr-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.⁸ A01H4/00, 7/00 |
| Abstract: | A high frequency (90.5%) of shoot bud induction was observed in mature zygotic embryos of Pinus kesiya Royle
ex Gord. that were pre-cultured in Quoirin and Lepoivre (LP) medium containing 23.15 μM Kn for 4 wk and then transferred to growth regulator-free medium. Multiplication and elongation of the shoot buds resulted in 1/2 LP medium containing 0.5 μM IBA and 4.5 μM BAP or Kn. Rooting was 72.3% in isolated shoots that were treated with
53.76 μMNAA for 24 h and then cultured on water-agar medium. Plantlets were hardened and successfully established on soil collected from pine forests with 70% survival. Biomass of the micropropagated plants was 3.2 times higher than seed-derived plants. |
| Page(s): | 262-266 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.06(2) [April 2007]
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