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http://nopr.niscair.res.in/handle/123456789/3018| Title: | Plantlet regeneration of Pinus kesiya Royle ex Gord. from mature embryos |
| Authors: | Tandon, Pramod Kumaria, Suman Choudhury, Hiranjit |
| Keywords: | growth characteristics;micropropagation;shoot buds induction;zygotic embryos;Pinus kesiya |
| Issue Date: | Apr-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.⁸ A01H4/00, 7/00 |
| Abstract: | A high frequency (90.5%) of shoot bud induction was observed in mature zygotic embryos of Pinus kesiya Royle ex Gord. that were pre-cultured in Quoirin and Lepoivre (LP) medium containing 23.15 μM Kn for 4 wk and then transferred to growth regulator-free medium. Multiplication and elongation of the shoot buds resulted in 1/2 LP medium containing 0.5 μM IBA and 4.5 μM BAP or Kn. Rooting was 72.3% in isolated shoots that were treated with 53.76 μMNAA for 24 h and then cultured on water-agar medium. Plantlets were hardened and successfully established on soil collected from pine forests with 70% survival. Biomass of the micropropagated plants was 3.2 times higher than seed-derived plants. |
| Page(s): | 262-266 |
| URI: | http://hdl.handle.net/123456789/3018 |
| ISSN: | 0972-5849 |
| Appears in Collections: | IJBT Vol.06(2) [April 2007] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 6(2) 262-266.pdf | 2.26 MB | Adobe PDF | View/Open |
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