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Title: Synthesis of ethyl oleate employing synthetic hydrogel-immobilized lipase of Bacillus coagulans MTCC-6375
Authors: Kanwar, S S
Kaushal, R K
Verma, M L
Kumar, Y
Azmi, W
Gupta, R
Chimni, S S
Chauhan, G S
Keywords: Poly (MAc-co-DMA-cl-MBAm)-immobilized lipase
synthetic hydrogel lipase
Bacillus coagulans MTCC-6375
ethyl oleate synthesis
Issue Date: Jan-2007
Publisher: CSIR
IPC CodeInt. Cl.⁸ C12N9/20, C12N11/04
Abstract: Ten polymeric hydrogels were chemically synthesized by varying the concentrations of copolymer (DMA) and cross-linker (MBAm) molecules. An alkaline lipase of Bacillus coagulans MTCC-6375 was immobilized onto a poly (MAc-co-DMA-cl-MBAm)-hydrogel support at pH 8.5 and temperature 55ºC in 16 h. The bound lipase possessed 7.6 U.g⁻¹ (matrix) lipase activity with a specific activity of 18 U.mg⁻¹ protein. Hydrogel bound-lipase catalyzed esterification of oleic acid and ethanol to synthesize ethyl oleate in n-nonane. Various kinetic parameters were optimized to produce ethyl oleate using immobilized lipase. The optimal parameters were bound enzyme/substrate (E/S) ratio 0.62 mg/mM, ethanol/oleic acid 100 mM:75 mM or 100 mM:100 mM, incubation time 18 h and reaction temperature 55ºC that resulted in approximately 53% conversion of reactants into ethyl oleate in n-nonane. However, addition of a molecular sieve to the reaction mixture promoted the conversion to 58% in 18 h in n-nonane, which was equivalent to 55 mM of ethyl oleate produced.
Page(s): 68-73
ISSN: 0972-5849
Source: IJBT Vol.06(1) [January 2007]

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