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Title: Analysis on the arcelin expression in bruchid pest resistant wild pulses using real time RT-qPCR
Authors: Sakthivelkumar, Shanmugavel
Veeramani, Velayutham
Hilda, Karuppiah
Arumugam, Munusamy
Janarthanan, Sundaram
Keywords: Antimetabolic protein;Canavalia virosa;Gene expression;Insects;Lablab purpureus;Pest resistance;Vigna umbellata
Issue Date: Dec-2014
Publisher: NISCAIR-CSIR, India
Abstract: Arcelin, the antimetabolic protein from wild pulses is a known natural insecticidal molecule. Wild pulses with high arcelin content could serve as potential source to increase the levels of insect resistance in cultivated pulse crops. In this study, arcelin (Arl) gene expression was screened in seven stored product insect pest resistant wild pulse varieties using real time RT-qPCR. Arcelin gene specific real time PCR primers were synthesized from arcelin mRNA sequence of the wild pulse variety, Lablab purpureus. The results revealed different levels of arcelin gene expression in the tested varieties. Canavalia virosa registered significantly high content indicating its suitability for utilization of arcelin gene in developing stored product insect pest resistance with other cultivated pulses.
Page(s): 1195-1200
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections: IJEB Vol.52(12) [December 2014]

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