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Title: Evaluation of ‘cattle’ and ‘Indian Bison’ type antigens of Mycobacterium avium subspecies paratuberculosis for diagnosis of Bovine Johne’s Disease using ‘indigenous ELISA’ and AGPT
Authors: Pahangchopi, Donna
Singh, Ran Vir
Singh, Shoor Vir
Das, Paritosh
Sharma, Deepak
Sardana, Tarun
Kumar, Naveen
Chaubey, Kundan Kumar
Gupta, Saurabh
Keywords: AGPT;BJD;ELISA;Indian cow;JD;Livestock;MAP;Tuberculosis
Issue Date: Dec-2014
Publisher: NISCAIR-CSIR, India
Abstract: Two antigens (‘cattle’ type and ‘Indian Bison’ type) of Mycobacterium avium subspecies paratuberculosis were evaluated for diagnosis of Johne’s disease (JD) in a gaushala (cattle herd). Of the 160 cows of Sahiwal and Hariana breeds screened, 81 (50.6%) tested positive in ELISA and 66 (41.8%) in AGPT test. Using the two antigens, 33.5% tested positive in both the tests while 41.1% tested negative. Exclusively, only 8.2% tested positive in ELISA while 17.1% tested positive in AGPT. Two antigens together detected 58.9% prevalence of MAP in the gaushala. Individually, indigenous ELISA using antigen from native source of MAP proved superior to AGPT in the diagnosis of JD in cows.
Page(s): 1182-1185
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections: IJEB Vol.52(12) [December 2014]

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